Lactobacillus

Lactobacillus

The culture media products from Ensenmi offer distinct advantages in the development and application of lactic acid bacteria fermentation. We can tailor specific fermentation culture media products to meet customer needs by considering differences in strains and fermentation processes. Below, we summarize the fermentation durations of lactic acid bacteria for different target products, their nitrogen requirements, and the key advantages of Ensenmi fermentation culture media products.

Fermentation Duration for Different Target Products

L-Lactic Acid Production: 16-36 hours

L-Phenylalanine Production: 24-48 hours, typically with the first 8-16 hours focusing on biomass growth and the later stage on substrate conversion/metabolite accumulation

Probiotic Biomass Production: 16-28 hours

Exopolysaccharide (EPS) Production: 24-72 hours

The fermentation duration for lactic acid bacteria is usually within 1-2 days, which means that in production, they need to be supplied with nitrogen sources that are more readily assimilated. In general, yeast extract can meet their fermentation requirements, while if EPS is the target product, yeast peptone is required for the fermentation process.

Nitrogen Requirements of Lactic Acid Bacteria

Lactic acid bacteria generally do not efficiently utilize simple inorganic nitrogen and require rich organic nitrogen sources, such as amino acids, peptides, or protein hydrolysates. Compared to corn steep liquor, selecting yeast extract, or yeast peptone according to fermentation duration offers more consistent batches and provides a richer supply of B vitamins, nucleotides, and a broader spectrum of amino acids.

Key Advantages of Ensenmi Fermentation culture media

Stable Organic Nitrogen Sources: 

Ensenmi yeast powder, yeast peptone, and yeast extract ensure consistent fermentation performance, reduce batch variability, and increase success rates.

B-Vitamin Enrichment: 

B vitamins in Ensenmi products act as cofactors for multiple enzymes, supporting sugar metabolism, amino acid metabolism, and energy conversion. This enhances glycolysis efficiency, improves substrate utilization, and stabilizes lactic acid production.

Nucleotide Supplementation: 

Nucleotides in Ensenmi products promote RNA/DNA synthesis, accelerate cell replication, and aid stress recovery, resulting in higher biomass activity, increased lactic acid accumulation, and improved substrate conversion.